phosphorylated iκb alpha  (Proteintech)

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Fibroblast Growth Factor 20 Attenuates Colitis by Restoring Impaired Intestinal Epithelial Barrier Integrity and Modulating Macrophage Polarization via S100A9 in an NF-κB-Dependent Manner

doi: 10.1016/j.jcmgh.2025.101486

Figure Lengend Snippet: FGF20 inhibits NF-κB pathway through S100A9 in DDS-induced mice. ( A ) KEGG Enrichment Analysis of proteomics data from the colonic tissue of FGF20 KO and WT mice with or without colitis. ( B ) Immunoblot analysis of colonic p-P65, P65, p-IκBα, and IκBα from FGF20 KO and WT mice with or without colitis (n = 6). ( C ) Immunoblot analysis of colonic p-P65, P65, p-IκBα, and IκBα contents in AAV-FGF20- or AAV-GFP-treated mice with or without colitis. ( D ) Immunoblot analysis of colonic p-P65, P65, p-IκBα, and IκBα contents in FGF20 KO mice and WT mice with colitis, treated with paquinimod or vehicle (n = 6). ( E ) Immunoblot analysis of colonic p-P65, P65, p-IκBα, and IκBα contents in AAV-FGF20-treated mice and AAV-GFP-treated mice with colitis, treated with AAV-S100A9 or not (n = 6). Data are represented as mean ± SEM; ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001.

Article Snippet: FGF20 (1/500, SC-373927, Santa CruzA), Bcl2 (1/1000, AB182858, Abcam), Bax (1/1000, AB32503, Abcam), S100A9 (1/500, SC-53187, Santa Cruz), S100A8 (1/500, SC-48352, Santa Cruz), phosphorylated (p) -IκBα (1/500, SC-8404, Santa Cruz), p-P65 (1/500, SC-136548, Santa Cruz), IκBα (1/1000, 4814S, CST, USA), P65 (1/1000, 4814S, CST), TNF-α (1/1000, AF7014, Affinity, China), IL6 (1/1000, 12912S, CST), FGFR1 (1/1000, bs-0230R, Bioss, China), FGFR2 (1/1000, bs-0675R, Bioss), FGFR3 (1/2000, MA5-31142, ThermoFisher), FGFR4 (1/1000, bsm-61509R, Bioss), GAPDH (1/10000, HRP-60004, Proteintech, China), and Beta-Tubulin (1/5000, 10094-1-AP, Proteintech).

Techniques: Western Blot

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Fibroblast Growth Factor 20 Attenuates Colitis by Restoring Impaired Intestinal Epithelial Barrier Integrity and Modulating Macrophage Polarization via S100A9 in an NF-κB-Dependent Manner

doi: 10.1016/j.jcmgh.2025.101486

Figure Lengend Snippet: FGF20 inhibits M1-like macrophage polarization in NF-κB-dependent manner in vitro. Primary peritoneal macrophages were isolated from WT mice and FGF20 KO mice and used for subsequent in vitro experiments. ( A ) Immunoblot analysis of expression of iNOS, TNF-α, IL6, S100A9, p-P65, P65, p-IκBα, and IκBα in LPS-stimulated primary peritoneal macrophages, treated with or without BAY11-7082 (n = 3). ( B ) Representative immunofluorescence images and its fluorescence densities of LPS-stimulated primary peritoneal macrophages, treated with or without BAY11-7082 (n = 6). Data are represented as mean ± SEM; ∗∗ P < .01, ∗∗∗ P < .001.

Article Snippet: FGF20 (1/500, SC-373927, Santa CruzA), Bcl2 (1/1000, AB182858, Abcam), Bax (1/1000, AB32503, Abcam), S100A9 (1/500, SC-53187, Santa Cruz), S100A8 (1/500, SC-48352, Santa Cruz), phosphorylated (p) -IκBα (1/500, SC-8404, Santa Cruz), p-P65 (1/500, SC-136548, Santa Cruz), IκBα (1/1000, 4814S, CST, USA), P65 (1/1000, 4814S, CST), TNF-α (1/1000, AF7014, Affinity, China), IL6 (1/1000, 12912S, CST), FGFR1 (1/1000, bs-0230R, Bioss, China), FGFR2 (1/1000, bs-0675R, Bioss), FGFR3 (1/2000, MA5-31142, ThermoFisher), FGFR4 (1/1000, bsm-61509R, Bioss), GAPDH (1/10000, HRP-60004, Proteintech, China), and Beta-Tubulin (1/5000, 10094-1-AP, Proteintech).

Techniques: In Vitro, Isolation, Western Blot, Expressing, Immunofluorescence, Fluorescence

Journal: Food Science & Nutrition

Article Title: Omega‐3 supplementation attenuates doxorubicin‐induced cardiotoxicity but is not related to the ceramide pathway

doi: 10.1002/fsn3.4492

Figure Lengend Snippet: Western blot analysis of nSMase1 expression (a) and total IkB, phosphorylated IkB, and total/phosphorylated ratio (b). C, control; D, doxorubicin; DW, doxorubicin + omega‐3 fatty acids. pD, value for doxorubicin effect; pW, value for omega‐3 fatty acids effect; pi, value doxorubicin + omega‐3 fatty acids interaction; W, omega‐3 fatty acids. Data are expressed as means ± SD. p , values are from two‐way analysis of variance. *Animals treated with doxorubicin were statistically different from animals not treated with chemotherapy. IkB and p‐IkB were incubated on the same membrane using stripping technique, and the same GADPH was used to normalize both.

Article Snippet: After electrophoresis, the proteins were transferred to nitrocellulose membrane and incubated with 5% skimmed milk blocking for 1 h. Afterward, the membrane was incubated with nSMase1 primary antibody (ab‐131330, ABCAM, Cambridge, UK), total IkBα (sc‐1643, Santa Cruz Biotechnology, Dallas, TX, USA), and phosphorylated IkBα (sc‐8404, Santa Cruz Biotechnology, Dallas, TX, USA) primary antibody, diluted in TBS / 3% skimmed milk overnight.

Techniques: Western Blot, Expressing, Control, Incubation, Membrane, Stripping Membranes